{"version":"oxtr-binding-outcomes-2026-10-21-v2","reviewed_on":"2026-10-21","scope":"Source-supported interpretation of the current mapped observations; source coordinates and measurements are unchanged.","priority":[{"label":"Binding detected at a defined sequence","description":"A positive binding result at a specified DNA sequence is the most direct anchor for a location comparison. Check the protein, assay and biological context."},{"label":"Protein association detected across a region","description":"ENCODE and BrainTF supply experimental support over a stretch of DNA. Resolve the site within it and inspect the contributing cell types and experiments."},{"label":"Sequence match predicted","description":"JASPAR matches are candidates for exploration and experimental follow-up. A model match alone does not establish binding at that location."}],"priority_note":"This is an Atlas research-priority guide, not a platform trust score. A published paper can contain any evidence type. Relevant context, assay quality and replication matter. Binding and effects on gene activity are separate outcomes.","geometry_note":"Biological support does not establish statistical significance or predictive value for an Atlas geometric match. That requires a defined comparison and appropriate controls.","negative_controls":"No negative binding controls have been curated for this coordinate experiment. An absent record or an unclassified outcome is not a negative experiment.","outcome_vocabulary":{"binding_detected":"Positive binding result reported for the named DNA element under the stated assay conditions.","regional_association_detected":"Positive regional protein-association signal; direct sequence-specific binding and an exact motif are not established by this cluster alone.","binding_not_detected":"No binding detected under specified assay conditions; assign only from an explicit source result.","mixed_or_unclear":"Results differ or are inconclusive; retain each assay and condition.","prediction_only":"The record reports a computational match; no location-specific binding result is established by this record.","not_classified":"Outcome not classified in this layer; not equivalent to untested or negative."},"defaults":{"studies":{"status":"not_classified","label":"Binding outcome not yet classified","resolution":"unresolved","result":"The outcome has not been classified in this interpretation layer; consult the original finding.","assay":"See source record","context":"See source record","expression_effect":"Not assessed in this interpretation layer","review_depth":"No outcome classification assigned","source_url":null},"oreganno":{"status":"not_classified","label":"Binding outcome not yet classified","resolution":"unresolved","result":"The outcome has not been classified in this interpretation layer; consult the original finding.","assay":"See source record","context":"See source record","expression_effect":"Not assessed in this interpretation layer","review_depth":"No outcome classification assigned","source_url":null},"encode":{"status":"regional_association_detected","label":"Regional association detected","resolution":"region","result":"A ChIP-seq peak cluster records protein association with this DNA region. Binding can be direct or mediated by other proteins; the plotted centre is not an identified binding site.","assay":"ChIP-seq peak clusters","context":"Contributing experiments and cell types are retained in the record.","expression_effect":"Effect on OXTR expression not established by this cluster","review_depth":"UCSC ENCODE 3 track methods and archived cluster provenance; individual raw experiments not reanalysed","source_url":"https://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg38&g=encRegTfbsClustered"},"jaspar":{"status":"prediction_only","label":"Prediction only","resolution":"motif_match","result":"A DNA sequence matches a JASPAR motif model. This record does not establish binding at this location in a cell.","assay":"Computational motif scan","context":"Model-derived sequence match; no cell-specific occupancy measurement in this record.","expression_effect":"Effect on OXTR expression not established by this prediction","review_depth":"JASPAR documentation and archived UCSC prediction","source_url":"https://jaspar.elixir.no/docs/"},"braintf":{"status":"regional_association_detected","label":"Regional association detected","resolution":"region","result":"A screened BrainTF ChIP-seq peak records protein association across this region; an exact binding sequence is unresolved.","assay":"ChIP-seq peak calls","context":"Human postmortem brain; the context is retained on each observation.","expression_effect":"Effect on native OXTR expression not established by this occupancy measurement.","review_depth":"Public processed peaks, author replication tests, FRiP and HOT annotations reviewed; raw reads not reanalysed.","source_url":"https://doi.org/10.1038/s41593-024-01658-8"}},"records":{"TFBS012":{"status":"binding_detected","label":"Binding detected","resolution":"reported_sequence","result":"US-1 showed protein binding in the reported DNA-binding assays. The specific binding protein remains unresolved.","assay":"Differential electrophoretic mobility shift assay (EMSA)","context":"Nuclear extracts from human term-pregnant and non-pregnant myometrium (uterine muscle).","expression_effect":"Enhancer activity reported in an artificial reporter construct in cultured SKN cells; not a direct measurement of the element’s effect at its native OXTR locus.","review_depth":"Published abstract reviewed; full-text methods and controls not independently reassessed","source_url":"https://pubmed.ncbi.nlm.nih.gov/10221779/"},"TFBS013":{"status":"binding_detected","label":"Binding detected","resolution":"reported_sequence","result":"US-2 showed specific binding by human MafF in the reported experiments.","assay":"Yeast one-hybrid screening followed by EMSA","context":"Human term-myometrium cDNA library and follow-up protein–DNA binding assays.","expression_effect":"A role in OXTR upregulation was proposed; a causal effect on native OXTR expression is not established by the reviewed abstract.","review_depth":"Published abstract reviewed; full-text methods and controls not independently reassessed","source_url":"https://pubmed.ncbi.nlm.nih.gov/10527846/"},"TFBS044":{"status":"regional_association_detected","label":"Regional association detected","resolution":"region","result":"A screened BrainTF ChIP-seq peak records protein association across this region; an exact binding sequence is unresolved.","assay":"ChIP-seq peak calls","context":"Human postmortem brain; the context is retained on each observation.","expression_effect":"Effect on native OXTR expression not established by this occupancy measurement.","review_depth":"Public processed peaks, author replication tests, FRiP and HOT annotations reviewed; raw reads not reanalysed.","source_url":"https://doi.org/10.1038/s41593-024-01658-8"},"TFBS045":{"status":"not_classified","label":"Functional reporter evidence; binding unresolved","resolution":"unresolved","result":"The outcome has not been classified in this interpretation layer; consult the original finding.","assay":"See source record","context":"See source record","expression_effect":"Human OCE7 DNA enhanced reporter activity in mouse cells and drove mouse olfactory-bulb reporter activity. Native human OXTR regulation remains unresolved.","review_depth":"No outcome classification assigned","source_url":"https://doi.org/10.1093/hmg/ddaf022"}}}