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Imaging translation in single cells using fluorescent microscopy.

Chao JA, Yoon YJ, Singer RH. · Cold Spring Harbor perspectives in biology · 2012 · Review

Original publication · Original publication · Original publication

Recorded summary

Reference associated with Tryptophan Autofluorescence as a Primary Optical Marker of Gene Transcription.

Recorded limitations

Bibliographic metadata matched; scientific claims await appraisal.

Evidence and provenance

Chapter Trust Factor uses a minimum of three distinct qualifying studies. Publication records retain their own evidence and assessment.

Study design and assessment
design
Review
assessment
Not recorded
trust Factor
scope
Paper
status
unassessed
score
Not recorded
basis
Bibliographic identity checks do not establish study design, blinding or evidential strength.
jadad
status
unassessed
score
Not recorded
evidence
Not recorded
Topics and recorded topic scores
  1. key
    general:autofluorescence-sensing
    title
    Autofluorescence and Molecular Sensing
    collection
    general
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    Not recorded
Citation snapshot and identifiers
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hsi-1bfd258cce7eef
doi
10.1101/cshperspect.a012310
pmid
22960595
citation Snapshot
Not recorded
citations By Collection
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Not recorded
Complete approved library record
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hsi-1bfd258cce7eef
aliases
None recorded
source References
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    NPL-197
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    hsi-1bfd258cce7eef
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    title
    Imaging translation in single cells using fluorescent microscopy.
    authors
    Chao JA, Yoon YJ, Singer RH.
    year
    2012
    journal
    Cold Spring Harbor perspectives in biology
    doi
    10.1101/cshperspect.a012310
    pmid
    22960595
    pmcid
    PMC3536344
    patent Number
    Not recorded
    original Assignee
    Not recorded
    original Citation
    Chao JA, Yoon YJ, Singer RH (2012). "Imaging Translation in Single Cells Using Fluorescent Microscopy." Cold Spring Harbor Perspectives in Biology 4(11):a012310 (PMC 3536344) — FUNCAT, fluorescent puromycin, fl-tRNA FRET, photoconvertible reporters, MS2/PP7 dual-label systems for single-molecule and single-cell translation imaging; establishes fluorescent microscopy as primary modality for visualising protein synthesis in vivo
    original Section
    Tryptophan Autofluorescence as a Primary Optical Marker of Gene Transcription
    metadata Status
    metadata-matched
    claim Review
    Not independently appraised
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    https://www.notion.so/3807b9c99c5680599be7d8337c817b15
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    topic Id
    autofluorescence-sensing
    role
    Focused chapter source
source Type
Review
source Verification
Bibliographic metadata matched; scientific claims await appraisal.
source Collection
Hyperspectral Imaging · patent section 2.4
title
Imaging translation in single cells using fluorescent microscopy.
journal
Cold Spring Harbor perspectives in biology
neurotransmitters
None recorded
year
2012
authors
Chao JA, Yoon YJ, Singer RH.
design
Review
kind
Review
identity Conflict
Not recorded
doi
10.1101/cshperspect.a012310
pmid
22960595
urls

https://pubmed.ncbi.nlm.nih.gov/22960595/ · https://doi.org/10.1101/cshperspect.a012310 · https://pmc.ncbi.nlm.nih.gov/articles/PMC3536344/

collections

general

categories

Hyperspectral Imaging

targets
None recorded
priority
1
quotes
None recorded
summary
Reference associated with Tryptophan Autofluorescence as a Primary Optical Marker of Gene Transcription.
limitation
Bibliographic metadata matched; scientific claims await appraisal.
citation Count
Not recorded
paper Trust Assessment
scope
Paper
status
unassessed
score
Not recorded
basis
Bibliographic identity checks do not establish study design, blinding or evidential strength.
citations By Collection
general
Not recorded
topics
  1. key
    general:autofluorescence-sensing
    title
    Autofluorescence and Molecular Sensing
    collection
    general
    trust Score
    Not recorded
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coldspringharborperspectivesinbiology
journal Label
Cold Spring Harbor perspectives in biology
chapter Entries
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    hsi-1bfd258cce7eef
    title
    Imaging translation in single cells using fluorescent microscopy.
    journal
    Cold Spring Harbor perspectives in biology
    year
    2012
    authors
    Chao JA, Yoon YJ, Singer RH.
    design
    Review
    urls

    https://pubmed.ncbi.nlm.nih.gov/22960595/ · https://doi.org/10.1101/cshperspect.a012310 · https://pmc.ncbi.nlm.nih.gov/articles/PMC3536344/

    quotes
    None recorded
    summary
    Reference associated with Tryptophan Autofluorescence as a Primary Optical Marker of Gene Transcription.
    related Versions
    None recorded
    limitation
    Bibliographic metadata matched; scientific claims await appraisal.
    citation Count
    Not recorded
    source Pages
    None recorded
    doi
    10.1101/cshperspect.a012310
    pmid
    22960595
    targets
    None recorded
    categories

    Hyperspectral Imaging

    source References
    1. ref
      NPL-197
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      hsi-1bfd258cce7eef
      source Type
      Review
      title
      Imaging translation in single cells using fluorescent microscopy.
      authors
      Chao JA, Yoon YJ, Singer RH.
      year
      2012
      journal
      Cold Spring Harbor perspectives in biology
      doi
      10.1101/cshperspect.a012310
      pmid
      22960595
      pmcid
      PMC3536344
      patent Number
      Not recorded
      original Assignee
      Not recorded
      original Citation
      Chao JA, Yoon YJ, Singer RH (2012). "Imaging Translation in Single Cells Using Fluorescent Microscopy." Cold Spring Harbor Perspectives in Biology 4(11):a012310 (PMC 3536344) — FUNCAT, fluorescent puromycin, fl-tRNA FRET, photoconvertible reporters, MS2/PP7 dual-label systems for single-molecule and single-cell translation imaging; establishes fluorescent microscopy as primary modality for visualising protein synthesis in vivo
      original Section
      Tryptophan Autofluorescence as a Primary Optical Marker of Gene Transcription
      metadata Status
      metadata-matched
      claim Review
      Not independently appraised
      source URL
      https://www.notion.so/3807b9c99c5680599be7d8337c817b15
      publication URL
      https://pubmed.ncbi.nlm.nih.gov/22960595/
      metadata URL
      https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=PMCID%3APMC3536344&format=json&resultType=core&pageSize=4
      corrections
      None recorded
      topic Id
      autofluorescence-sensing
      role
      Focused chapter source
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    Review
    source Verification
    Bibliographic metadata matched; scientific claims await appraisal.
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    Autofluorescence and Molecular Sensing
    trust Score
    Not recorded
    paper Trust Assessment
    scope
    Paper
    status
    unassessed
    score
    Not recorded
    basis
    Bibliographic identity checks do not establish study design, blinding or evidential strength.
jadad
status
unassessed
score
Not recorded
condition Evidence
None recorded
conditions
None recorded
topic Trust Scores
None recorded
full Text
status
not-indexed
retrieval Available
false

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