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Protein Fluorescence

Joseph R. Lakowicz · Principles of Fluorescence Spectroscopy · 1999 · Book or chapter

Original publication

Recorded summary

Reference associated with Tryptophan Autofluorescence as a Primary Optical Marker of Gene Transcription.

Recorded limitations

Bibliographic metadata matched; scientific claims await appraisal.

Evidence and provenance

Chapter Trust Factor uses a minimum of three distinct qualifying studies. Publication records retain their own evidence and assessment.

Study design and assessment
design
Book or chapter
assessment
Not recorded
trust Factor
scope
Paper
status
unassessed
score
Not recorded
basis
Bibliographic identity checks do not establish study design, blinding or evidential strength.
jadad
status
unassessed
score
Not recorded
evidence
Not recorded
Topics and recorded topic scores
  1. key
    general:autofluorescence-sensing
    title
    Autofluorescence and Molecular Sensing
    collection
    general
    trust Score
    Not recorded
Citation snapshot and identifiers
id
hsi-507bbc03f5d2eb
doi
10.1007/978-1-4757-3061-6_16
pmid
Not recorded
citation Snapshot
Not recorded
citations By Collection
general
Not recorded
Complete approved library record
id
hsi-507bbc03f5d2eb
aliases
None recorded
source References
  1. ref
    NPL-188
    record Id
    hsi-507bbc03f5d2eb
    source Type
    Book or chapter
    title
    Protein Fluorescence
    authors
    Joseph R. Lakowicz
    year
    1999
    journal
    Principles of Fluorescence Spectroscopy
    doi
    10.1007/978-1-4757-3061-6_16
    pmid
    Not recorded
    pmcid
    Not recorded
    patent Number
    Not recorded
    original Assignee
    Not recorded
    original Citation
    Lakowicz JR (2006). Principles of Fluorescence Spectroscopy. 3rd ed. Springer. Ch. 16: "Protein Fluorescence" — Tryptophan indole ring (fused hexagonal benzene + pentagonal pyrrole) as dominant intrinsic protein fluorophore; excitation ~280 nm, emission ~340 nm; π→π* transitions of delocalised electrons across aromatic ring geometry; quantum yield sensitivity to local microenvironment; >90% of total protein UV fluorescence from tryptophan; molar extinction coefficient ~5,500 M⁻¹cm⁻¹ at 280 nm
    original Section
    Tryptophan Autofluorescence as a Primary Optical Marker of Gene Transcription
    metadata Status
    metadata-matched
    claim Review
    Not independently appraised
    source URL
    https://www.notion.so/3807b9c99c5680599be7d8337c817b15
    publication URL
    https://doi.org/10.1007/978-1-4757-3061-6_16
    metadata URL
    https://api.crossref.org/works?query.title=Protein+Fluorescence&rows=3
    corrections
    None recorded
    topic Id
    autofluorescence-sensing
    role
    Focused chapter source
source Type
Book or chapter
source Verification
Bibliographic metadata matched; scientific claims await appraisal.
source Collection
Hyperspectral Imaging · patent section 2.4
title
Protein Fluorescence
journal
Principles of Fluorescence Spectroscopy
neurotransmitters
None recorded
year
1999
authors
Joseph R. Lakowicz
design
Book or chapter
kind
Book or chapter
identity Conflict
Not recorded
doi
10.1007/978-1-4757-3061-6_16
pmid
Not recorded
urls

https://doi.org/10.1007/978-1-4757-3061-6_16

collections

general

categories

Hyperspectral Imaging

targets
None recorded
priority
1
quotes
None recorded
summary
Reference associated with Tryptophan Autofluorescence as a Primary Optical Marker of Gene Transcription.
limitation
Bibliographic metadata matched; scientific claims await appraisal.
citation Count
Not recorded
paper Trust Assessment
scope
Paper
status
unassessed
score
Not recorded
basis
Bibliographic identity checks do not establish study design, blinding or evidential strength.
citations By Collection
general
Not recorded
topics
  1. key
    general:autofluorescence-sensing
    title
    Autofluorescence and Molecular Sensing
    collection
    general
    trust Score
    Not recorded
journal Key
principlesoffluorescencespectroscopy
journal Label
Principles of Fluorescence Spectroscopy
chapter Entries
  1. id
    general-autofluorescence-sensing-hsi-507bbc03f5d2eb
    record Id
    hsi-507bbc03f5d2eb
    title
    Protein Fluorescence
    journal
    Principles of Fluorescence Spectroscopy
    year
    1999
    authors
    Joseph R. Lakowicz
    design
    Book or chapter
    urls

    https://doi.org/10.1007/978-1-4757-3061-6_16

    quotes
    None recorded
    summary
    Reference associated with Tryptophan Autofluorescence as a Primary Optical Marker of Gene Transcription.
    related Versions
    None recorded
    limitation
    Bibliographic metadata matched; scientific claims await appraisal.
    citation Count
    Not recorded
    source Pages
    None recorded
    doi
    10.1007/978-1-4757-3061-6_16
    pmid
    Not recorded
    targets
    None recorded
    categories

    Hyperspectral Imaging

    source References
    1. ref
      NPL-188
      record Id
      hsi-507bbc03f5d2eb
      source Type
      Book or chapter
      title
      Protein Fluorescence
      authors
      Joseph R. Lakowicz
      year
      1999
      journal
      Principles of Fluorescence Spectroscopy
      doi
      10.1007/978-1-4757-3061-6_16
      pmid
      Not recorded
      pmcid
      Not recorded
      patent Number
      Not recorded
      original Assignee
      Not recorded
      original Citation
      Lakowicz JR (2006). Principles of Fluorescence Spectroscopy. 3rd ed. Springer. Ch. 16: "Protein Fluorescence" — Tryptophan indole ring (fused hexagonal benzene + pentagonal pyrrole) as dominant intrinsic protein fluorophore; excitation ~280 nm, emission ~340 nm; π→π* transitions of delocalised electrons across aromatic ring geometry; quantum yield sensitivity to local microenvironment; >90% of total protein UV fluorescence from tryptophan; molar extinction coefficient ~5,500 M⁻¹cm⁻¹ at 280 nm
      original Section
      Tryptophan Autofluorescence as a Primary Optical Marker of Gene Transcription
      metadata Status
      metadata-matched
      claim Review
      Not independently appraised
      source URL
      https://www.notion.so/3807b9c99c5680599be7d8337c817b15
      publication URL
      https://doi.org/10.1007/978-1-4757-3061-6_16
      metadata URL
      https://api.crossref.org/works?query.title=Protein+Fluorescence&rows=3
      corrections
      None recorded
      topic Id
      autofluorescence-sensing
      role
      Focused chapter source
    source Type
    Book or chapter
    source Verification
    Bibliographic metadata matched; scientific claims await appraisal.
    collection
    general
    chapter Number
    11
    topic Id
    autofluorescence-sensing
    topic Title
    Autofluorescence and Molecular Sensing
    trust Score
    Not recorded
    paper Trust Assessment
    scope
    Paper
    status
    unassessed
    score
    Not recorded
    basis
    Bibliographic identity checks do not establish study design, blinding or evidential strength.
jadad
status
unassessed
score
Not recorded
condition Evidence
None recorded
conditions
None recorded
topic Trust Scores
None recorded
full Text
status
not-indexed
retrieval Available
false

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