Introduction to Fluorescent Proteins
Institutional, commercial or web source
Recorded summary
Reference associated with Tryptophan Autofluorescence as a Primary Optical Marker of Gene Transcription.
Recorded limitations
Documentary source retained; publication status and claims await appraisal.
Evidence and provenance
Chapter Trust Factor uses a minimum of three distinct qualifying studies. Publication records retain their own evidence and assessment.
Study design and assessment
- design
- Institutional, commercial or web source
- assessment
- Not recorded
- trust Factor
- scope
- Paper
- status
- unassessed
- score
- Not recorded
- basis
- Bibliographic identity checks do not establish study design, blinding or evidential strength.
- jadad
- status
- unassessed
- score
- Not recorded
- evidence
- Not recorded
Topics and recorded topic scores
- key
- general:autofluorescence-sensing
- title
- Autofluorescence and Molecular Sensing
- collection
- general
- trust Score
- Not recorded
Citation snapshot and identifiers
- id
- hsi-d9b2c705e7f695
- doi
- Not recorded
- pmid
- Not recorded
- citation Snapshot
- Not recorded
- citations By Collection
- general
- Not recorded
Complete approved library record
- id
- hsi-d9b2c705e7f695
- aliases
- None recorded
- source References
- ref
- NPL-199
- record Id
- hsi-d9b2c705e7f695
- source Type
- Institutional, commercial or web source
- title
- Introduction to Fluorescent Proteins
- authors
- Not recorded
- year
- Not recorded
- journal
- Not recorded
- doi
- Not recorded
- pmid
- Not recorded
- pmcid
- Not recorded
- patent Number
- Not recorded
- original Assignee
- Not recorded
- original Citation
- Piston DW, Campbell RE, Day RN, Davidson MW. "Introduction to Fluorescent Proteins." ZEISS Microscopy Online Campus — Education in Microscopy and Digital Imaging, Carl Zeiss Microscopy (zeiss-campus.magnet.fsu.edu/articles/probes/fpintroduction.html) — Comprehensive tutorial on the structure, spectral properties, brightness, maturation, and oligomerisation of fluorescent proteins derived from Aequorea victoria GFP and Anthozoa reef corals; details the 11-stranded β-barrel scaffold that protects the chromophore; the self-catalysed tripeptide cyclisation and oxidation reaction forming extended π-electron conjugated systems responsible for fluorescence; chromophore engineering via site-directed mutagenesis; fusion chimera design; fluorescent protein reporters and biosensors for monitoring gene expression, protein kinase activity, ion concentration, voltage, and cyclic nucleotide signalling in living cells
- original Section
- Tryptophan Autofluorescence as a Primary Optical Marker of Gene Transcription
- metadata Status
- documentary-source
- claim Review
- Not independently appraised
- source URL
- https://www.notion.so/3807b9c99c5680599be7d8337c817b15
- publication URL
- http://zeiss-campus.magnet.fsu.edu/articles/probes/fpintroduction.html
- metadata URL
- Not recorded
- corrections
Institutional, product or web reference; no journal-publication identity is asserted.
- topic Id
- autofluorescence-sensing
- role
- Focused chapter source
- source Type
- Institutional, commercial or web source
- source Verification
- Documentary source retained; publication status and claims await appraisal.
- source Collection
- Hyperspectral Imaging · patent section 2.4
- title
- Introduction to Fluorescent Proteins
- journal
- neurotransmitters
- None recorded
- year
- Not recorded
- authors
- design
- Institutional, commercial or web source
- kind
- Institutional, commercial or web source
- identity Conflict
- Not recorded
- doi
- Not recorded
- pmid
- Not recorded
- urls
http://zeiss-campus.magnet.fsu.edu/articles/probes/fpintroduction.html
- collections
general
- categories
Hyperspectral Imaging
- targets
- None recorded
- priority
- 1
- quotes
- None recorded
- summary
- Reference associated with Tryptophan Autofluorescence as a Primary Optical Marker of Gene Transcription.
- limitation
- Documentary source retained; publication status and claims await appraisal.
- citation Count
- Not recorded
- paper Trust Assessment
- scope
- Paper
- status
- unassessed
- score
- Not recorded
- basis
- Bibliographic identity checks do not establish study design, blinding or evidential strength.
- citations By Collection
- general
- Not recorded
- topics
- key
- general:autofluorescence-sensing
- title
- Autofluorescence and Molecular Sensing
- collection
- general
- trust Score
- Not recorded
- journal Key
- journal Label
- chapter Entries
- id
- general-autofluorescence-sensing-hsi-d9b2c705e7f695
- record Id
- hsi-d9b2c705e7f695
- title
- Introduction to Fluorescent Proteins
- journal
- year
- Not recorded
- authors
- design
- Institutional, commercial or web source
- urls
http://zeiss-campus.magnet.fsu.edu/articles/probes/fpintroduction.html
- quotes
- None recorded
- summary
- Reference associated with Tryptophan Autofluorescence as a Primary Optical Marker of Gene Transcription.
- related Versions
- None recorded
- limitation
- Documentary source retained; publication status and claims await appraisal.
- citation Count
- Not recorded
- source Pages
- None recorded
- doi
- Not recorded
- pmid
- Not recorded
- targets
- None recorded
- categories
Hyperspectral Imaging
- source References
- ref
- NPL-199
- record Id
- hsi-d9b2c705e7f695
- source Type
- Institutional, commercial or web source
- title
- Introduction to Fluorescent Proteins
- authors
- Not recorded
- year
- Not recorded
- journal
- Not recorded
- doi
- Not recorded
- pmid
- Not recorded
- pmcid
- Not recorded
- patent Number
- Not recorded
- original Assignee
- Not recorded
- original Citation
- Piston DW, Campbell RE, Day RN, Davidson MW. "Introduction to Fluorescent Proteins." ZEISS Microscopy Online Campus — Education in Microscopy and Digital Imaging, Carl Zeiss Microscopy (zeiss-campus.magnet.fsu.edu/articles/probes/fpintroduction.html) — Comprehensive tutorial on the structure, spectral properties, brightness, maturation, and oligomerisation of fluorescent proteins derived from Aequorea victoria GFP and Anthozoa reef corals; details the 11-stranded β-barrel scaffold that protects the chromophore; the self-catalysed tripeptide cyclisation and oxidation reaction forming extended π-electron conjugated systems responsible for fluorescence; chromophore engineering via site-directed mutagenesis; fusion chimera design; fluorescent protein reporters and biosensors for monitoring gene expression, protein kinase activity, ion concentration, voltage, and cyclic nucleotide signalling in living cells
- original Section
- Tryptophan Autofluorescence as a Primary Optical Marker of Gene Transcription
- metadata Status
- documentary-source
- claim Review
- Not independently appraised
- source URL
- https://www.notion.so/3807b9c99c5680599be7d8337c817b15
- publication URL
- http://zeiss-campus.magnet.fsu.edu/articles/probes/fpintroduction.html
- metadata URL
- Not recorded
- corrections
Institutional, product or web reference; no journal-publication identity is asserted.
- topic Id
- autofluorescence-sensing
- role
- Focused chapter source
- source Type
- Institutional, commercial or web source
- source Verification
- Documentary source retained; publication status and claims await appraisal.
- collection
- general
- chapter Number
- 11
- topic Id
- autofluorescence-sensing
- topic Title
- Autofluorescence and Molecular Sensing
- trust Score
- Not recorded
- paper Trust Assessment
- scope
- Paper
- status
- unassessed
- score
- Not recorded
- basis
- Bibliographic identity checks do not establish study design, blinding or evidential strength.
- jadad
- status
- unassessed
- score
- Not recorded
- condition Evidence
- None recorded
- conditions
- None recorded
- topic Trust Scores
- None recorded
- full Text
- status
- not-indexed
- retrieval Available
- false