Universal Informatics · Research reading room

Effect of 528 Hz Music on the Endocrine System and Autonomic Nervous System

Health · 2018 · Small participant-masked crossover study

Original publication · Original publication

Recorded summary

After five minutes of 528 Hz music, mean salivary oxytocin rose from 37.57 to 73.58 and cortisol later fell from 0.43 to 0.25; neither change was significant with 440 Hz music.

Attributed extracts from the research record

“ In the 528 Hz condition , mean levels of cortisol significantly decreased , chromogranin A tended to decrease , and oxytocin doubled after only 5 minutes of music exposure . However, no significant change was observed in any salivary biomarkers in the 440 Hz condition.”
The influence of music on the autonomic nervous system and endocrine system varies depending on the frequency of the music, and furthermore, that 528 Hz music has an especially strong stress-reducing effect , even following only five minutes of exposure .”

Recorded limitations

Only nine healthy adults participated. The short comparison is intriguing biomarker evidence, not clinical proof, and requires larger independent preregistered replication.

Evidence and provenance

Chapter Trust Factor uses a minimum of three distinct qualifying studies. Publication records retain their own evidence and assessment.

Study design and assessment
design
Small participant-masked crossover study
assessment
Not recorded
trust Factor
score
Not recorded
scope
Paper
status
unassessed
randomized Controlled Trial
Not recorded
blinding
not-confirmed
peer Reviewed
false
basis
Publication-specific design or publication status remains unconfirmed.
sources

https://www.scirp.org/pdf/Health_2018090514214117.pdf

review Scope
Existing publication-specific assessments and bibliographic indexing; no blanket full-text appraisal.
assessed At
2026-10-27
jadad
status
unassessed
score
Not recorded
evidence
Not recorded
Topics and recorded topic scores
  1. key
    sound:ii
    title
    528 Hz sound: gene expression, hormonal responses and cell studies
    collection
    sound
    trust Score
    Not recorded
Citation snapshot and identifiers
id
src-ae3dc0d94fef
doi
10.4236/health.2018.109088
pmid
Not recorded
citation Snapshot
Not recorded
citations By Collection
sound
4
Complete approved library record
id
src-ae3dc0d94fef
publication Key
Not recorded
aliases

portal-75ae9eff6740

title
Effect of 528 Hz Music on the Endocrine System and Autonomic Nervous System
journal
Health
neurotransmitters
None recorded
year
2018
authors
design
Small participant-masked crossover study
kind
Source record
identity Conflict
Not recorded
doi
10.4236/health.2018.109088
urls

https://www.scirp.org/pdf/Health_2018090514214117.pdf · https://doi.org/10.4236/health.2018.109088

collections

sound

categories

Molecular biomarkers · Heart rate & autonomic

targets

OXT

priority
1
quotes

“ In the 528 Hz condition , mean levels of cortisol significantly decreased , chromogranin A tended to decrease , and oxytocin doubled after only 5 minutes of music exposure . However, no significant change was observed in any salivary biomarkers in the 440 Hz condition.” · The influence of music on the autonomic nervous system and endocrine system varies depending on the frequency of the music, and furthermore, that 528 Hz music has an especially strong stress-reducing effect , even following only five minutes of exposure .”

summary
After five minutes of 528 Hz music, mean salivary oxytocin rose from 37.57 to 73.58 and cortisol later fell from 0.43 to 0.25; neither change was significant with 440 Hz music.
limitation
Only nine healthy adults participated. The short comparison is intriguing biomarker evidence, not clinical proof, and requires larger independent preregistered replication.
citation Count
4
paper Trust Assessment
score
Not recorded
scope
Paper
status
unassessed
randomized Controlled Trial
Not recorded
blinding
not-confirmed
peer Reviewed
false
basis
Publication-specific design or publication status remains unconfirmed.
sources

https://www.scirp.org/pdf/Health_2018090514214117.pdf

review Scope
Existing publication-specific assessments and bibliographic indexing; no blanket full-text appraisal.
assessed At
2026-10-27
citations By Collection
sound
4
topics
  1. key
    sound:ii
    title
    528 Hz sound: gene expression, hormonal responses and cell studies
    collection
    sound
    trust Score
    Not recorded
journal Key
health
journal Label
Health
chapter Entries
  1. id
    ii-src-ae3dc0d94fef
    record Id
    src-ae3dc0d94fef
    title
    Effect of 528 Hz Music on the Endocrine System and Autonomic Nervous System
    journal
    Health
    year
    2018
    authors
    design
    Small participant-masked crossover study
    urls

    https://www.scirp.org/pdf/Health_2018090514214117.pdf · https://doi.org/10.4236/health.2018.109088

    quotes
      1. text
        “
        emphasis
        false
      2. text
        In the 528 Hz condition
        emphasis
        true
      3. text
        , mean levels of
        emphasis
        false
      4. text
        cortisol significantly decreased
        emphasis
        true
      5. text
        ,
        emphasis
        false
      6. text
        chromogranin A tended to decrease
        emphasis
        true
      7. text
        , and
        emphasis
        false
      8. text
        oxytocin doubled after only 5 minutes of music exposure
        emphasis
        true
      9. text
        . However, no significant change was observed in any salivary biomarkers in the 440 Hz condition.”
        emphasis
        false
      1. text
        The influence of music on the autonomic nervous system and endocrine system varies depending on the frequency of the music, and furthermore, that
        emphasis
        false
      2. text
        528 Hz music has an especially strong stress-reducing effect
        emphasis
        true
      3. text
        , even
        emphasis
        false
      4. text
        following only five minutes of exposure
        emphasis
        true
      5. text
        .”
        emphasis
        false
    citation Count
    4
    source Pages

    15

    targets

    OXT

    categories

    Molecular biomarkers · Heart rate & autonomic

    doi
    10.4236/health.2018.109088
    pmid
    Not recorded
    aliases

    portal-75ae9eff6740

    summary
    After five minutes of 528 Hz music, mean salivary oxytocin rose from 37.57 to 73.58 and cortisol later fell from 0.43 to 0.25; neither change was significant with 440 Hz music.
    limitation
    Only nine healthy adults participated. The short comparison is intriguing biomarker evidence, not clinical proof, and requires larger independent preregistered replication.
    summary Source
    name
    I AM portal
    url
    https://doi.org/10.4236/health.2018.109088
    reviewed At
    2026-08-15
    paper Trust Assessment
    score
    Not recorded
    scope
    Paper
    status
    unassessed
    randomized Controlled Trial
    Not recorded
    blinding
    not-confirmed
    peer Reviewed
    false
    basis
    Publication-specific design or publication status remains unconfirmed.
    sources

    https://www.scirp.org/pdf/Health_2018090514214117.pdf

    review Scope
    Existing publication-specific assessments and bibliographic indexing; no blanket full-text appraisal.
    assessed At
    2026-10-27
    collection
    sound
    chapter Number
    1
    topic Id
    ii
    topic Title
    528 Hz sound: gene expression, hormonal responses and cell studies
    trust Score
    Not recorded
jadad
status
unassessed
score
Not recorded
condition Evidence
None recorded
conditions
None recorded
topic Trust Scores
None recorded
full Text
status
not-indexed
retrieval Available
false

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